WITNESS RELAXIN (Canine/Feline Pregnancy Test)
Relaxin can be detected in biological samples soon after implantation of the fertilized egg, which occurs 20-31 days post-LH surge, or approximately 16-33 days post-mating.
� Early detection of planned or unplanned pregnancy
� Detection of pregnancy before or after abortion treatment
� Early detection of false pregnancy
� Diagnose fetal resorption or spontaneous abortion
� No refrigeration required
� Results available within 10 minutes
� Plasma or serum samples
� Rapid immuno migration (unidirectional lateral flow) stat test format
WITNESS� RELAXIN
Zoetis
CANINE AND FELINE PREGNANCY TEST KIT
I. GENERAL INFORMATION
WITNESS� RELAXIN is intended to determine pregnancy in the bitch and queen, as well as to distinguish between pseudopregnancy and real gestation in the bitch. The test measures Relaxin levels in plasma and serum samples. The presence of significant amounts of this hormone is a reliable indicator of pregnancy.
The WITNESS� RELAXIN test provides an early, inexpensive and reliable way to determine success or failure of a planned mating or unwanted exposure.
II. PREGNANCY DETECTION
Canine: Relaxin can be detected in biological samples soon after implantation of the fertilized egg, which occurs about 18 days after LH surge. Relaxin is first detectable by WITNESS� RELAXIN during the fourth week of pregnancy (from D22 to D28 after ovulation), reaches its highest level in the 6th-8th weeks, and then declines prior to parturition.
Feline: Some pregnancies may be detected as early as 25 days post-mating, while others may not test positive until after 31 days. It is recommended to test after this date if a previous result was negative.
Occasionally, false positive results may occur in non-pregnant queens with elevated Relaxin levels due to cystic ovaries.
III. TEST PRINCIPLE
WITNESS� RELAXIN is a simple test based on Rapid Immuno Migration (RIM�) technology that uses the combination of two anti-Relaxin antibodies to quickly identify this hormone in biological samples (serum or plasma) from the bitch and queen.
Colloidal gold particles sensitized with an anti-Relaxin antibody bind to Relaxin molecules present in the sample.
The formed complex migrates along a membrane. The complex is then captured on a sensitized reaction line (second antibody) where its accumulation causes the formation of a clearly visible pink/red band.
A pink/red band in the control window (3) ensures that the test was performed correctly.
IV. SAMPLE COLLECTION
� The test can be performed on serum or plasma (anticoagulated with EDTA, sodium citrate or heparin).
� Always collect samples with a sterile needle and syringe.
� Hemolysis does not significantly interfere with the test, but strongly hemolyzed samples may partly obscure a weak positive band.
V. SAMPLE STORAGE
� Samples should preferably be tested immediately after collection but not longer than 4 hours after collection, if stored at room temperature.
� If testing is further delayed, samples should be kept refrigerated (up to 2 days at +2 �C to 8 �C; 35 �F to 46 �F).
� For long term storage, samples should be kept frozen (-20 �C; -4 �F or colder).
VI. KIT CONTENTS
� 5 Pouches, each containing 1 test device and desiccant.
� 5 Pipettes.
� 1 Saline Buffer bottle (2 mL).
� Instructions for use.
VII. PRECAUTIONS
� Do not use this kit or any of its components after the expiration date.
� Kit should be stored at +2 �C - 25 �C (35 �F - 77 �F). Kit should not be frozen.
� Use the test immediately after the pouch is first opened (within 10 minutes).
� Avoid touching or damaging the membrane in the sample well or the results windows.
� The WITNESS� device should be placed on a flat, horizontal surface while performing the test.
� Use a separate pipette for each sample.
� Hold pipette and buffer bottle vertically when dispensing sample and buffer respectively.
� Handle all samples as biohazardous material.
� For veterinary use only.
